Avengers (1963) #229
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Earth's Mightiest Heroes joined forces to take on threats that were too big for any one hero to tackle. With a roster that has included Captain America, Iron Man, Ant-Man, Hulk, Thor, Wasp and dozens more over the years, the Avengers have come to be regarded as Earth's No. 1 team.
After brilliant scientist Henry Pym discovered a process in which he could manipulate a newly discovered group of subatomic particles through magnetic fields, he tested the serums on himself and accidentally shrunk himself to the size of an ant.