The Amazing Spider-Man (1963) #91
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Peter Parker's first true love, Gwen Stacy was killed during a rescue attempt by Spider-Man, the guilt from which Peter has never overcome.
Bitten by a radioactive spider, high school student Peter Parker gained the speed, strength and powers of a spider. Adopting the name Spider-Man, Peter hoped to start a career using his new abilities. Taught that with great power comes great responsibility, Spidey has vowed to use his powers to help people.