Marvel Team-Up (1972) #59
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Characters
Bitten by a radioactive spider, high school student Peter Parker gained the speed, strength and powers of a spider. Adopting the name Spider-Man, Peter hoped to start a career using his new abilities. Taught that with great power comes great responsibility, Spidey has vowed to use his powers to help people.
When Janet Van Dyne's father died, she convinced her father's associate Hank Pym to give her a supply of "Pym particles"; Pym also subjected her to a procedure which granted her the ability to, upon shrinking, grow wings and fire blasts of energy, which she called her "wasp's stings."
After brilliant scientist Henry Pym discovered a process in which he could manipulate a newly discovered group of subatomic particles through magnetic fields, he tested the serums on himself and accidentally shrunk himself to the size of an ant.